...Ultra™ Directional RNA Library Prep Kit for Illumina...

来自 : 发布时间：2024-05-10

Accept cookiesWe use cookies to understand how you use our site and to improve the overall user experience. This includes personalizing content and advertising. To learn more and manage cookies, please refer to our Cookie Statement.Are you doing COVID-19 related research? Our latest RUO kit, the Luna SARS-CoV-2 RT-qPCR Multiplex Assay Kit, enables high throughput workflows for real-time detection of SARS-CoV-2 nucleic acid using hydrolysis probes. For simple, visual assay results, the SARS-CoV-2 Rapid Colorimetric LAMP Assay Kit includes a color-changing pH indicator for detection of SARS-CoV-2 nucleic acid amplificationLearn about our tools that are helping researchers develop diagnostics and vaccines for the SARS-CoV-2 virus.Time for ChangeMonarch Nucleic Acid Purification Kits are optimized for maximum performance and minimal environmental impact. Kits are available for total RNA purification, plasmid miniprep, gel extraction, and DNA RNA cleanup. For maximum convenience and value, columns and buffers are also available separately. Learn more and request a sample!The small size of this product was discontinued on 12/31/2020. The large size will continue to be available.This original version of the Ultra Directional RNA Library Prep Kit enables strand-specific RNA library preparation in a streamlined workflow. However, we now recommend the new NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB #E7760), which has been further optimized to provide increased yields of high quality libraries from a broader range of input amounts.To find out how to order this product from your current location, click the button below: International Customer Order Support The NEBNext Ultra Directional RNA Library Prep Kit for Illumina contains reagents for preparation of strand-specific RNA libraries for Illumina sequencing. Please note that adaptors, primers, rRNA depletion reagents and poly(A) mRNA isolation reagents are not included in the kit and are available separately.Each kit component must pass rigorous quality control standards, and each set of reagents is functionally validated together by construction and sequencing of a library on the Illumina sequencing platform.Ultra Directional RNA Library Preparation for IlluminaNEBNext Ultra Directional RNA produces librarieswith high directionality/strand specificityUltra Directional RNA libraries of mRNA purified from Universal Human Reference RNA (Agilent #740000), were sequenced on the Illumina platform. The high percentage of reads mapped to theexpected strand shows the high strand-specificity of the libraries. Actinomycin D is required toachieve maximal strand specificity.NEBNext Ultra Directional RNA libraries provide evencoverage across transcriptsmRNA was isolated from Universal Human Reference RNA (Agilent # 740000) and libraries were madeusing the NEBNext Ultra Directional RNA Kit and the Illumina TruSeq Stranded mRNA Kit. Librarieswere sequenced (single end reads) on the Illumina platform (GAIIx). Good coverage across expressedtranscripts was obtained.Low input NEBNext Ultra Directional RNA librariesretain complexityRibosomal RNA-depleted RNA libraries were madefrom 10 ng or 100 ng Universal Human Reference RNA(Agilent #740000) using the NEBNext Ultra DirectionalRNA Kit. Transcript abundances in Fragments PerKilobase of exon per Million fragments mapped(FPKM) show strong concordance (R2 = 0.90745),indicating retention of library complexity even at lowinput amount.Equivalent transcript representation is achievedbetween technical replicates of NEBNext UltraDirectional RNA librariesTechnical replicates of mRNA libraries made frommRNA isolated from Universal Human ReferenceRNA (Agilent #740000) show strong concordance(R2 = 0.99409).Sequencing data shows strong concordance betweenNEBNext Ultra Directional RNA and IlluminaTruSeq Stranded RNA librariesmRNA was isolated from Universal Human ReferenceRNA (Agilent #740000) and libraries were made usingthe NEBNext Ultra Directional RNA Kit and the IlluminaTruSeq Stranded mRNA Kit. This FPKM concordanceplot shows comparable transcript representation(R2 = 0.96009) between the libraries.Lot ControlThe lots provided are managed separately and qualified by additional functional validation. Individual reagents undergo standard enzyme activity and quality control assays, and also meet stringent criteria in the additional quality controls listed on each individual component page Kit ComponentsThe following reagents are supplied with this product:Features Accurate retention of transcript strand of origin information Utilizes dUTP-based methodology Low input amounts (as low as 10ng purified mRNA or ribosomal-depleted RNA, or 100ng Total RNA) Fast workflow (4.5 5 hours), with minimal hands-on time Robust, reliable performance Ultra high fidelity amplification with minimized GC biasNEBNext Poly(A) mRNA Magnetic Isolation Module (NEB #E7490)or NEBNext rRNA Depletion Kit (NEB #E6310)NEBNext Singleplex (NEB #E7350) or NEBNext Multiplex (NEB #E7335, #E7500, #E7710, #E7730, #E6609,#E7600) Oligos for Illumina or customer supplied OligosMagnetic rack or plate (e.g., NEBNext Magnetic Separation Rack (NEB #S1515S), Alpaqua 96S Super Magnet Plate (#A001322), or equivalent)80% Ethanol (freshly prepared)0.1X TE, pH 8.010 mM Tris-HCl, pH 7.5 8.010 mM NaCl (optional)Agencourt AMPure XP Beads (Beckman Coulter, Inc. #A63881)Actinomycin D (Sigma# A1410, dissolved in dimethylsulfoxide [DMSO] to 5 g/ul)Additional required materials not included for use with NEBNext rRNA Depletion Kit (NEB #E6310): Agencourt RNAClean XP (Beckman Coulter, Inc., Cat #A63987) The Product Manual includes details for how to use the product, as well as details of its formulation and quality controls. manualE7420 Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.SpecificationsThe Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version] E7420S_L_v1 This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.For more information about commercial rights, please contact NEB\'s Global Business Development team at gbd@neb.com.This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.New England Biolabs (NEB) is committed to practicing ethical science we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more. LicensesThis product is covered by one or more patents.This product is licensed for research and commercial use from Bio-Rad Laboratories, Inc., under U.S. Pat. Nos. 6,627,424, 7,541,170, 7,670,808, 7,666,645, and corresponding patents in other countries. No rights are granted for use of the product for Digital PCR or real-time PCR applications, with the exception of quantification in Next Generation Sequencing workflows.This product and its use are the subject of one or more issued and/or pending U.S. and foreign patent applications owned by Max Planck Gesellschaft. The purchase of this product from New England Biolabs, Inc., its affiliates, or its authorized resellers and distributors conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product in research conducted by the buyer (whether the buyer is an academic or for profit entity) or for limited commercial purposes that are pre-approved by New England Biolabs, Inc. The purchase of this product does not convey a license under any claims in the foregoing patents or patent applications directed to producing the product. The buyer cannot sell or otherwise transfer this product or its components to a third party or otherwise use this product for the following COMMERCIAL PURPOSES: (1) use of the products or its components in manufacturing; (2) use of the product or its components for therapeutic or prophylactic purposes in humans or animals.For additional information or to inquire about commercial use, please contact busdev@neb.com.Ineligible item added to cartBased on your Freezer Program type, you are trying to add a product to your cart that is either not allowed or not allowed with the existing contents of your cart. Please review and update your order accordingly If you have any questions, please contact Customer Service at freezers@neb.com or 1-800-632-5227 x 8.If you don t see your country above, please visit ourinternational siteSession ExpiredYou have been idle for more than 20 minutes, for your security you have been logged out. Please sign back in to continue your session.Sign InYour profile has been mapped to an Institution, please sign back for your profile updates to be completed.Sign InTo save your cart and view previous orders, sign in to your NEB account. Adding products to your cart without being signed in will result in a loss of your cart when you do sign in or leave the site.