A Simple Portable Electroluminescence Illumination-Based CCD...

来自 : 发布时间：2024-05-10

A Simple Portable Electroluminescence Illumination-Based CCD Detector 2014-06-03 13:38 In this chapter we describe a simple and relatively inexpensive Electroluminescence (EL) illumination and charged-coupled device (CCD) camera (EL-CCD) based detector for monitoring florescence and colorimetric assays. The portable battery-operated florescence detector includes an EL panel for fluoro-genic excitation at 490 nm, a cooled CCD digital camera to monitor emission at 523 nm, filters and a close up lens. The detector system is controlled by a laptop computer for camera operation, image acquisition and analysis. The system was tested using a fluorogenic peptide substrate (SNAP-25) for botulinum neurotoxin serotype A (BoNT-A) labeled with FITC. The level of detection of the system was found to be 1.25 nM of the peptide, similar to the detection level of a commercial photomultipler-based plate fluorometer. The multichannel EL-CCD was used with an assay plate capable of testing nine samples simultaneously in 1 min at this detection level. The portable system is small and is operated by a 12 V source. The modular detector was designed with easily interchangeable ELs, filters and lenses and can be used and adapted for a wide variety of florescence and colorimetric assays, florescence labels and assay formats.超方便的实验干货查询工具 微信扫码进入「丁香实验」小程序 查看全部 来源：互联网 超方便的实验干货查询工具微信扫码进入「丁香实验」 知道了 你可能喜欢 活体荧光成像系统（图）