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Illumina/Ribo-Zero rRNA Removal Kit (Gram-Positive Bacteria) 6 reactions 99% of rRNA from 1-5 µg of Gram-positive bacter
ProductHighlights:
- RemovesribosomalRNA(rRNA)fromintactandpartiallydegradedGram(+)bacteria
- SequencingdatacontainscompletetranscriptomeofcodingandnoncodingRNAspecies
- Goodforsmallsamplequantities–only1μgoftotalRNArequired
- NuclearrRNAremoved:23S,16S,5S
Sequencingisanimportantdiscoverytoolforresearchintodisease,development,andmore.ThesequencingdatafromsamplestreatedwiththeRibo-ZerorRNARemovalKit(Gram-PositiveBacteria)arefocusedonvaluableRNAspecies,tofacilitatediscoveryofrelevantgenes,splicevariants,andisoforms.
TheRibo-ZerorRNARemovalKit(Gram-PositiveBacteria)offersinformativesequencingresultsbyremovingunwantedrRNApriortosequencing.ThekitremovesrRNAfrombothintactandpartiallydegradedsamples.
RemovesallmajorrRNAsubunits
TheRibo-ZerorRNARemovalKit(Gram-PositiveBacteria)depletesunwantedrRNAfromtotalRNA.RemovaloftheseunwantedsubunitsproducessequencingresultsthatareheavilyfocusedonBIOLOGicallyinformativegenomicregions.
OverviewoftheRibo-Zeroprocess
VirtuallyallrRNAisdepletedin2hours.
Step1:Washthemagneticbeads
Step2:TreatsampleswithrRNARemovalSolution
Step3:RemoverRNA
Step4:PurifyrRNA-depletedsamples,andproceedwithlibrarypreparation
Alternativekitversions
TheRibo-ZerorRNARemovalKit(Bacteria)isrecommendedforsamplescomposedofmixturesofGram-negativeandGram-positivebacteria.
RelatedInformation
rRNARemovalKitSelectionGuide
Ribo-ZeroKitSpeciesCompatibility
Ribo-ZeroKitFAQs
