ProductHighlights:
The24sure+microarrayisdesignedtoimprovethesuccessofinvitrofertilization(IVF)bydetectingsubchromosomalimbalancesinembryosofreciprocaltranslocationcarriers.24sure+includesincreasedcoverageofpericentromericandsubtelomericregionstoensuretheidentificationandreportingofsmallderivedchromosomefragmentsfromsinglecells.
24sure+microarraysofferseveralkeyadvantages:
- 24sure+canmoreaccuratelydeterminebalancedoreuploidembryosforselectioncomparedtotrADItionalfluorescentinsituhybridization(FISH)methods1
- Rapidworkflowenablessampleresultsandreportingwithin12hours
- Every24sure+purchaseincludesBlueFuseMultisoftwareforthefullyautomatedanalysis,interpretation,andreportingof24sure+investigations
24sure+analysisisideallyofferedtocouplesundergoingIVFduetorecurrentmiscarriagessUSPectedtobecausedbythepresenceofabalancedtranslocationineitherparent.Balancedtranslocationcarriersmaynotpresentwithaphenotype.However,segregationofthetranslocatedchromosomesduringgameteformationcanproducearangeofnormal,balanced,andunbalancedgametes,withunbalancedproductsexpectedtorepresent50%ofgametes.2Resultingembryoswithlargestructuralimbalancesarelikelytobenon-viable.Asaresult,thebalancedcarriermaypresentwithinfertilityorahistoryofrecurrentmiscarriage/spontaneousabortion.3
Couplespresentingwithrecurrentmiscarriagecanbekaryotyped,whichisalow-resolutionanalysisthatmaydeterminewhethertheproblemstheyareexperiencingareduetoabalancedrearrangement.Ifthisisthecase,suchcarriersofbalancedtranslocationsmayseek24sure+analysisofembryos.
Specifications:
| AssayTime | ~12hours |
| Hands-OnTime | ~3.5hours |
| InputQuantity | SurePlexwhole-genome-amplifiedsinglecellsfromembryobiopsies |
| Technology | Microarray |
| Method | PGDArray |
| SpecializedSampleTypes | SingleCells |
| SpeciesCategory | Human |
| VariantClass | ChromosomalAbnormalities |
