ProductHighlights:
TheOvineSNP50BeadChiparrayfeaturesover54,241evenlyspacedprobesthattargetSNPs,offeringmorethansufficientSNPdensityforgenome-wideassociationstudiesandotherapplicationssuchasgenome-wideselection,determinationofgeneticmerit,identificationofquantitativetraitloci,andcomparativegeneticstudies.
TheBeadChipwasdevelopedincollaborationwithleADIngovineresearchersfromAgResearch,BaylorUCSC,CSIRO,andtheUSDAaspartoftheInternationalSheepGenomicsConsortium.Itfeaturesover54,241evenlyspacedprobesthattargetsinglenucleotidepolymorphisms(SNPs).
Morethan18,000oftheseMarkerswerediscoveredthroughsequencingreducedrepresentationlibrarieswiththeIlluminaGenomeAnalyzerIIx.Asetof600SNPswereidentifiedbyBACendsequencingandvalidatedwithIlluminaGoldenGateGenotypingAssays,forover403animalsfrom23breeds.TheremainingSNPswerederivedfromthedraftovinegenome.
TheOvineSNP50BeadChipprovidesuniformgenome-widecoveragewithanestimatedonemarkerper46kbonaverage.TheBeadChipprovideshighcallrates,allowsforflexIBLecontentdeployment,andenablesthedetectionandmeasurementofcopynumbervariation.
- TheBeadChipfurtherreducesexperimentalvariABIlitybyallowingresearcherstointerrogateupto12samplesinparallel
- Theassay"ssingle-tubesamplepreparationwithoutPCRorligationstepssignificantlyreduceslaborandpotentialsamplehandlingerrors
LearnMoreAboutPlantandAnimalGenotyping
Specifications:
| InputQuantity | 200ngDNA |
| NumberofMarkers | Fixedmarkers:54,241evenlydistributedSNPsacrossthegenomeu003cbru003eCustommarkeradd-oncapacity:None |
| Method | Genome-WideGenotypingArray |
| Technology | Microarray |
| SpecializedSampleTypes | FFPE |
| SpeciesCategory | Ovine |
| AutomationCapability | LiquidHandlingRobots,Autoloader2.xArrayLoader |
| SystemCompatibility | iScan |
