ProductHighlights:
WiththeInfiniumMethylationEPICBeadChipKit,researcherscaninterrogateover850,000methylationsitesquantitativelyacrossthegenomeatsingle-nucleotideresolution.Multiplesamples,includingFFPE,canbeanalyzedinparalleltodeliverhigh-throughputpowerwhileminimizingthecostpersample.
InfiniumHDarraytechnologyenablescontentselectionindependentofbias-associatedlimitationsoftenassociatedwithmethylatedDNAcapturemethods.Theresultisapan-enhancerandcodingregionviewofthemethylomethatcanbeusedforepigenome-wideassociationstudiesonavarietyofhumantissues.
ComprehensiveGenome-WideCoverage
Thiskitincludesthefollowingcontentcategoriesrequestedbymethylationexperts:
- CpGsitesoutsideofCpGislands
- Non-CpGmethylatedsitesidentifiedinhumanstemcells(CHHsites)
- Differentiallymethylatedsitesidentifiedintumorversusnormal
- FANTOM5enhancers
- ENCODEopenchromatinandenhancers
- DNasehypersensitivesites
- miRNApromoterregions
- >90%ofcontentcontainedontheIlluminaHumanMethylation450KBeadChip
IntegratedAnalysisSoftware
MethylationEPICdataanalysisissupportedbytheGenomeStudioMethylationModule.GenomeStudiosoftwareenablesresearcherstoeffortlesslyperformdifferentialmethylationanalysisforsmall-scalestudies.Itfeaturesadvancedvisualizationtoolsthatenableresearcherstoviewvastamountsofdatainasinglegraph,suchasheatmaps,scatterplots,andlineplots.
ThesetoolsandtheGenomeStudioGenomeBrowserdisplayvaluableinformationsuchaschromosomalcoordinates,percentGC,locationinaCpGIsland,andmethylationβvalues.
Forlarge-scalestudies,therearemanyfreeanalysispackagesthatworkinthesoftwareframeworkRfornormalization,anddifferentialanalysisofmethylationdata.Instructionalvideosexplaintheuseofthesedataanalysispackages.
Specifications:
| NumberofMarkers | Over850,000methylationsitespersampleatsingle-nucleotideresolution |
| InputQuantity | 250ngDNA |
| Technology | Microarray |
| SpecializedSampleTypes | FFPE,LowInput |
| VariantClass | DifferentiallyMethylatedCytosines |
| SpeciesCategory | Human |
| SystemCompatibility | iScan |
