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DNA_library_preparation >
Illumina/TruSeq绞合mRNA文库制备试剂盒套装A（48个样本，12个索引）/RS-122-2101/1 Ea
ProductHighlights:
TheTruSeqStrandedmRNALibraryPrepKitoffersastreamlined,cost-efficient,andscalablesolutionforcodingtranscriptomeanalysis.ItiscompatIBLewithawiderangeofsamples.
PreciseandAccurate
GetprecisemeasurementofmRNAstrandorientationfordetectionofantisensetranscription,enhancedtranscriptannotation,andincreasedalignmentefficiency.Highcoverageuniformityenhancesthediscoveryoffeaturessuchasalternativetranscripts,genefusions,andallele-specificexpression.
Cost-Efficient
Strandedinformationidentifiesfromwhichofthe2DNAstrandsagivenRNAtranscriptwasderived.Thisinformationprovidesincreasedconfidenceintranscriptannotation,particularlyfornonhumansamples.Identifyingstrandoriginincreasesthepercentageofreadsthatalign,reducingsequencingcostspersample.
Scalable
Thekitenablesrobustinterrogationofbothstandardandlow-qualitysamples,andworkflowscompatiblewithawiderangeofstudydesigns.
Findanup-to-datelistofhigh-throughputautomationvendorswithroboticsystemscompatiblewiththislibrarypreparationkit
Specifications:
| AssayTime | ~10.5hours |
| Hands-OnTime | ~4.5hours |
| InputQuantity | 0.1–1ugtotalRNAor10-100ngpreviouslyisolatedmRNA(fromspecieswithpolyAtails) |
| ContentSpecifications | Capturesthecodingtranscriptomewithstrandinformation |
| MechanismofAction | Oligo-dTbeadscapturepolyAtails |
| SystemCompatibility | NovaSeq5000,GenomeAnalyzerIIx,HiSeq2000,MiSeq,NextSeq550,HiSeq3000,HiSeq1000,HiSeq1500,NextSeq500,HiSeq2500,NovaSeq6000,HiSeq4000 |
| SpeciesCategory | Other,Mammalian,Bovine,Mouse,Human,Rat |
| VariantClass | SingleNucleotidePolymorphisms(SNPs),GeneFusions,NovelTranscripts,TranscriptVariants |
| Technology | Sequencing |
| Method | mRNASequencing |
| AutomationCapABIlity | LiquidHandlingRobots |
