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Illumina/TruSeq Stranded Total RNA Library Prep Kit with Ribo-Zero Globin Set B (48 samples, 12 indexes)/RS-122-2502/1 E
ProductHighlights:
TruSeqStrandedTotalRNAwithRibo-ZeroGlobinkitsdeliveraclearandcomprehensiveviewofthetranscriptomefromblood-derivedRNA,withafast,efficientsequencinglibrarypreparationworkflow.
High-qualitywhole-transcriptomeanalysiswithprecisestrandinformation
ThesekitscouplethebenefitsofRibo-ZeroribosomalRNAreductionchemistrywithRNAsequencing(RNA-Seq)technologyforwhole-transcriptomeanalysisofhuman,mouse,orratsamples.Thistechnologyprovides:
- Uniformcoverageandprecisemeasurementofstrandorientation
- High-confidencediscoveryofalternativetranscripts,antisenseexpression,andallele-specificexpression
- Discoveryacrossbothcodingandmultipleformsofnon-codingRNA
EfficientribosomalRNAandglobinmRNAremovalinasinglestep
Whole-transcriptomeanalysisofblood-derivedRNArequirestheremovaloftwoformsofabundantRNA—ribosomalRNA(bothcytoplasmicandmitochondrial)aswellasglobinmRNA,whichispresentinhighlevelsinwholeblood.
TrADItionalremovalmethodsrequiretwoindependentsteps.Thismeanstheneedforadditionalreagents,alongerworkflow,andmoreinputRNAlost.TruSeqStrandedTotalRNAwithRibo-ZeroGlobinleveragesRibo-ZerochemistrytoefficientlyremovebothformsofabundantRNAinasingle,rapidstep.
Findanup-to-datelistofhigh-throughputautomationvendorswithroboticsystemscompatIBLewiththislibrarypreparationkit
Specifications:
| InputQuantity | 0.1–1ughigh-qualitypurifiedtotalRNAfromblood |
| ContentSpecifications | CapturescodingRNAplusmultipleformsofnon-codingRNA |
| MechanismofAction | Bead-basedrRNAdepletion,CDNAsynthesis,andPCR |
| Multiplexing | Low-throughputkits:Poolupto12samples.Orpoolupto24sampleswithsetsAandBtogether,High-throughputkitversion:Prepare96uniquelyindexedsamples |
| SystemCompatibility | NovaSeq5000,GenomeAnalyzerIIx,HiSeq2000,NextSeq500,HiSeq2500,NextSeq550,HiSeq3000,HiSeq1000 |
| AutomationCapABIlity | LiquidHandlingRobots |
| VariantClass | TranscriptVariants,SingleNucleotidePolymorphisms(SNPs),GeneFusions |
| SpeciesCategory | Rat,Mouse |
| Method | Whole-TranscriptomeSequencing |
| Technology | Sequencing |
