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Illumina/TruSeq绞合总RNA文库制备试剂盒，具有核糖零人/小鼠/大鼠高通量（96份样本，96份索引
ProductHighlights:
TheTruSeqStrandedTotalLibraryPreparationkitprovidesaclearviewofthetranscriptomewithastreamlined,cost-efficient,andscalablesolutionfortotalRNAanalysiswithsequencing.
Whole-TranscriptomeAnalysiswithPreciseMeasurementofStrandOrientation
CompatIBLewithawiderangeofsamples,includinglow-qualityandFFPE,TruSeqStrandedTotalRNAkitscoupleallofthebenefitsofTruSeqRNApreparationkitswithRibo-ZeroribosomalRNAreductionchemistry,providinganalysisofcodingandmultipleformsofnoncodingRNAwithprecisemeasurementofstrandorientation,uniformcoverage,andhigh-confidencediscoveryoffeaturessuchasalternativetranscripts,genefusions,andallele-specificexpression.
Findanup-to-datelistofhigh-throughputautomationvendorswithroboticsystemscompatiblewiththislibrarypreparationkit
FlexibleSolutionsforaRangeofStudyDesigns
TheTruSeqStrandedTotalRNALibraryPrepkitisavailableinmultipleversionstofitspecificstudydesignneeds:
- TruSeqStrandedTotalRNAwithRibo-ZeroHuman/Mouse/RatremovescytoplasmicrRNA.
- TruSeqStrandedTotalRNAwithRibo-ZeroGoldremovesbothcytoplasmicandmitochondrialrRNA.
- ThetotalRNAkitsarealsocompatiblewiththenewuniquedualindexkits,TruSeqRNAUniqueDualIndexes,whichallowsmultiplexingofupto96samples.
SpeciesCompatibility
TheTruSeqStrandedTotalLibraryPreparationkitisdesignedforhuman,mouseandratsequences,butmaybeapplicabletoavarietyofeukaryoticspecies.*
*Forinformationonaparticularspeciesofinterest,contactIlluminaTechnicalSupport.
Specifications:
| InputQuantity | 0.1–1ughigh-qualitytotalRNA.Lower-qualitysamplesmayrequirefurtheroptimization. |
| ContentSpecifications | CapturescodingRNAplusmultipleformsofnon-codingRNA |
| MechanismofAction | Bead-basedrRNAdepletion,CDNAsynthesis,andPCR |
| Multiplexing | Low-throughputkits:Poolupto12samples.Orpoolupto24sampleswithsetsAandBtogether,High-throughputkits:Prepare96uniquelyindexedsamples. |
| SystemCompatibility | NovaSeq5000,GenomeAnalyzerIIx,HiSeq2000,NextSeq550,HiSeq3000,HiSeq1000,HiSeq1500,NextSeq500,HiSeq2500,NovaSeq6000,HiSeq4000 |
| VariantClass | SingleNucleotidePolymorphisms(SNPs),GeneFusions,NovelTranscripts,TranscriptVariants |
| SpecializedSampleTypes | FFPE |
| Method | Whole-TranscriptomeSequencing |
| SpeciesCategory | Mouse,Human,Rat |
| Technology | Sequencing |
| AutomationCapABIlity | LiquidHandlingRobots |
