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用于NeoPrep的Illumina/TruSeq纳米DNA文库准备工具包（16个索引，16个样本）/NP-101-1001/1 Ea
ProductHighlights:
TheTruSeqNanoDNAforNeoPrepoffersasimple,all-inclusiveprepsolution,idealforanywhole-genomesequencingapplication.RunthesameprovenTruSeqNanoDNAbiochemistryonthefullyintegrateddigitalmicrofluidicsNeoPrepLibraryPrepSystem.
- Simplifiedandseamlessworkflowsolution–Includesonboardquantificationandnormalizationtodeliversequencing-readylibraries
- Exceptionalperformanceandreproducibility–GenerateslibrarieswithTruSeqNanoDNAcoverageandquality,withminimalhands-ontimeandreduceduservariABIlity
- Lowinputrequirement–Requiresaslittleas25nggenomicDNAtopreparehigh-quality,sequencing-readylibraries
ReduceLibraryBiasandCoverageGaps
TruSeqNanoDNAlibraryprepreducesthenumberandaveragesizeoftypicalPCR-inducedgapsincoverage.Theenhancedworkflowreduceslibrarybiasandimprovescoverageuniformityacrossthegenome.ItalsoprovidesexcellentcoverageoftrADItionallychallenginggenomiccontent,includingGC-richregions,promoters,andrepetitiveregions.Thisenablesyoutoaccessmoreinformationfromeachsequencingrun.
NeoPrepSystemenhancesTruSeqNanoDNAperformance
TheNeoPrepSystemenhancesTruSeqNanoDNAperformancebyprovidinghigh-qualityreproducIBLeresults,evenwithlowinputamountsofDNA.Digitalmicrofluidicstechnologypreciselymanipulatesdropletsthatperformthelibraryprepworkflow,includingquantificationandnormalizationwithinthetightlycontrolledenvironmentoftheNeoPreplibrarycard.
Asimple,intuitiveworkflowdelivers16sequencing-readylibraries,eliminatingalmostallmanualsteps,andreducinghands-ontimefrom~4hourstojust30minutes.Inaddition,digitalmicrofluidicsrequireslessDNAinput,enablingexcellentperformancefrom25-75ngofgenomicDNA.SuccessfullibrarieshavebeendemonstratedwithDNAinputsrangingfrom1–100ng,upto10-foldlowerthanrequiredbymanualprotocols.
Specifications:
| AssayTime | 1day |
| Hands-OnTime | 4hours |
| InputQuantity | 50ngRNA,50nghigh-qualitytotalRNA,≥200ngFFPEtotalRNA;Recommendedquantitymayvarywithexpressionlevel,targetplexity,andsamplequality |
| ContentSpecifications | Choosefrom400,000+pre-designedtargetedRNA-Seqassays.Oraddcontenttoafixedpanelorpreviouslydesignedcustompanel. |
| Multiplexing | Upto384samplespersequencingrun |
| MechanismofAction | Amplification |
| Method | ShotgunSequencing,Whole-GenomeSequencing,GenotypingbySequencing |
| VariantClass | SingleNucleotidePolymorphisms(SNPs),GeneFusions,LossofHeterozygosity(LOH),SomaticVariants,ChromosomalAbnormalities,GermlineVariants,StructuralVariants,Insertions-Deletions(indels),CopyNumberVariants(CNVs) |
| SpeciesCategory | Mammalian,Mouse,Human,Other,Rat,Plant |
| SystemCompatibility | NextSeq550,HiSeq3000,HiSeqXFive,HiSeq1000,MiSeqDxinResearchMode,MiniSeq,HiSeq2000,MiSeq,HiSeqXTen,NeoPrep,HiSeq1500,NextSeq500,HiSeq2500,HiSeq4000 |
| SpecializedSampleTypes | LowInput |
| Technology | Sequencing |
| AutomationCapability | NeoPrepDigitalMicrofluidics |
