ProductHighlights:
TruSeqChIPLibaryPreparationKitsprovideasimple,cost-effectivesolutionforgeneratingchromatinimmunoprecipitationsequencing(ChIP-Seq)librariesfromChIP-derivedDNA.ChIP-Seqleveragesnext-generationsequencing(NGS)toquicklyandefficientlydeterminethedistributionandabundanceofDNA-boundproteintargetsofinterestacrossthegenome.
ThismethodhasbecomeoneofthemostwidelyusedNGSapplications,enablingresearcherstoreliablyandsimultaneouslyidentifybindingsitesofabroadrangeoftargetsacrosstheentiregenome,withhighresolutionandwithoutconstraints.
TruSeqChIPLibraryPreparationKitsarecompatIBLewithallIlluminasequencers.OfferingtheprovendataqualityandeaseofuseofTruSeqDNAsequencing,theyprovideastreamlinedChIP-Seqlibrarypreparationworkflowthatleveragesreagentmastermixestominimizepipettingandreducetotalassaytime.
ThekitshavealowDNAinputrequirementandarecompatiblewitharangeofsamplesources.Inaddition,theyoffermorerobustmultiplexsequencingwith24uniqueindexes,allowingresearcherstooptimizethedistributionofsequencingoutputacrosssamplesbasedonreaddepthrequirements.
Specifications:
| AssayTime | ~6.5hours |
| Hands-OnTime | ~3.5hours |
| InputQuantity | 10nghigh-qualitygenomicDNA;10–100ngFFPEDNA(dependingonQCresults) |
| MechanismofAction | Probehybridization,extension-ligation,andPCR |
| Multiplexing | 1–96 |
| ContentSpecifications | Designcustomprobestosequencegenomicregionsofinterest.Contentrange:4–650kb |
