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当前位置: 首页 > 产品中心 > DNA_library_preparation > Illumina/Nextera Mate配对库准备工具包(12个索引,48个无凝胶样品或12个凝胶样品)/FC-132-1001/1个
商品详细Illumina/Nextera Mate配对库准备工具包(12个索引,48个无凝胶样品或12个凝胶样品)/FC-132-1001/1个
Illumina/Nextera Mate配对库准备工具包(12个索引,48个无凝胶样品或12个凝胶样品)/FC-132-1001/1个
Illumina/Nextera Mate配对库准备工具包(12个索引,48个无凝胶样品或12个凝胶样品)/FC-132-1001/1个
商品编号: FC-132-1001
品牌: Illumina
市场价: ¥59516.60
美元价: 45782.00
产地: 美国(厂家直采)
公司:
产品分类: DNA文库制备试剂盒
公司分类: DNA_library_preparation
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

ProductHighlights:

TheNexteraMatePairLibraryPreparationKitisanidealapproachfordenovosequencing,genomefinishing,andthedetectionofstructuralvariation.ItiscompatIBLewithlargeDNAgenomes—eventhemostcomplexgenomessuchasthosederivedfromcancer.

  • SequenceprecioussampleswhenlimitedDNAisavailable
  • Generatehighlydiverselibrarieswithpremierdataquality
  • Preparesequencing-readylibrariesinlessthantwodays
Requires10TimesLessDNAInputThanOtherProtocols

Bothgel-freeandgel-plusprotocolsareavailable:

  • Thegel-freeprotocolallowsmatepairsequencingwhenlimitedDNAisavailable.Itisdesignedforapplicationssuchasdenovoassemblyofsmallgenomes.
  • Thegel-plusprotocolisidealforchallengingmatepairapplications,suchasdenovoassemblyofcomplexgenomes.
IncreasedLibraryDiversityandDataQuality

Thekitprovidesidentifiablejunctionsequencesforaccurateidentificationofthetwohalvesofthematepairfragment.

  • OnlybiotinylatesDNAmoleculesatfragmentationsites,avoidingtroublesomeinternalbiotinylation
  • Allowsforthecreationofmillionsofuniquefragments
  • Increasedlibrarydiversitygeneratesfewerduplicatereadsandpremierdataquality
FastandSimpleMatePairWorkflow

TheNexteraMatePairkitusesTruSeqDNALibraryPreparationmaster-mixedreagents,therebyreducingthenumberofassaystepsandhands-ontimerequired.

  • Fewerpipettingstepssimplifiesworkflowandreducessampleloss
  • Gel-freeprotocoloptionandon-beadreactionssimplifypurificationstepsandshortenprotocolhands-ontime

Specifications:

AssayTime1.5-2days
Hands-OnTime1.5-2hours
InputQuantity1ugDNAforgel-freeprotocol,4ugDNAforgel-plusprotocol
MultiplexingCanmultiplexupto12samples,dependingongenomesize.CompatiblewithmostlargeDNAgenomes.
MechanismofActionMethodsthattargetthejunctionsiteoflargecircularizedfragments.Mechanicalfragmentation(COVARIS).
SystemCompatibilityMiSeq,HiSeq3000,HiSeq2500,HiSeq4000
SpeciesCategoryOther,Mammalian,Mouse,Human,Rat,Plant,Bacteria
VariantClassSingleNucleotidePolymorphisms(SNPs),StructuralVariants,Insertions-Deletions(indels)
MethodWhole-GenomeSequencing,DeNovoSequencing,Long-ReadSequencing
TechnologySequencing
品牌介绍
基因组服务Illumina提供了多样化的服务组合,以支持广泛的基因组应用。无论您是进行基于人群的大规模基因分析研究,进行癌症研究还是推进育种计划,Illumina和我们的合作伙伴都能为您提供所需的解决方案。借助久经考验的Illumina NGS和阵列技术以及科学专家网络,客户越来越有能力加快发现机会。Illumina服务网络提供了一个从样品到数据的解决方案,可以保证交付高质量的数据,从而帮助您以前所未有的速度和效率找到答案。Illumina还提供许多其他服务,包括核心实验室计划,服务合作伙伴关系,分析和数据管理解决方案以及财务解决方案。