ProductHighlights:
TheForenSeqDNASignaturePrepKitispartofacomplete,fullyvalidatedDNA-to-datasolutiondesignedforforensicgenomicsapplications.Itincludesallreagentstopreparenext-generationsequencing(NGS)librariesfromforensicDNAsamples.
Achievehighresolutionandexceptionalaccuracyfromaslittleas1ngofDNA—EvenwithcomplexmixturesordegradedDNA.Thiskitallowslabsto:
- Performmultiplexingandrapidsampleprocessing
- Accessawiderrangeofinformativesingle-nucleotidepolymorphisms(SNPs)withasinglekit
- Analyzechallengingsamples
Multiplexingandrapidsampleprocessing
TheForenSeqDNASignaturePrepKitsupportspreparationofupto96librariessimultaneouslyusingasimpleplate-basedformatandstandardlabequipment.Targetedprimermixesenableanalysisofautosomal,Y-andX-chromosomeshorttandemrepeat(STR)targets,andidentity-informativeSNPs,allinasinglereaction.
Optionally,youcanincludebiogeographicalancestry-informativeSNPs(aiSNPs)andphenotypic-informativeSNPs(piSNPs).Thisdatacanbecriticalingeneratinginvestigativeleadsfrom“nosUSPect”casesthathaveotherwisegonecold.
AwiderrangeofinformativeSNPswithasinglekit
TheForenSeqDNASignaturePrepKitconsolidatesallautosomalSTRMarkerscurrentlyusedaroundtheworldforcaseworkandcriminalDNAdatabases.Thissingle,streamlinedworkfloweliminatestheneedtorunmultipleSTRtests.
Thekitdeliversapproximately200geneticmarkersinasingletest,removingthetradeoffsandriskimposedbytechnicallimitationssuchaslowDNAquantity.Italsocontainsadensesetofidentityinformativesinglenucleotidepolymorphism(iiSNP)markersetsnotroutinelyavailablewithtrADItionalcapillaryelectrophoresis(CE)methods.
Superioranalysisofchallengingsamples
CasesareoftencomplicatedandsometimesunresolvedduetothepresenceofhighlydegradedDNA,lowqualityDNA,orcomplexDNAmixtures.TheForenSeqDNASignaturePrepKitincludesalargenumberofmarkers,manyofwhicharehighlypolymorphic.
Theseadditionalmarkers,coupledwiththeinherentsensitivityofIlluminachemistry,helpdetectminorcomponentsthatmightgoundetectedbyconventionalSTRandCEanalysis.
Specifications:
| AssayTime | ~6.5hours |
| Hands-OnTime | ~3.5hours |
| InputQuantity | 10nghigh-qualitygenomicDNA;10–100ngFFPEDNA(dependingonQCresults) |
| MechanismofAction | Probehybridization,extension-ligation,andPCR |
| Multiplexing | 1–96 |
| ContentSpecifications | Designcustomprobestosequencegenomicregionsofinterest.Contentrange:4–650kb |
| SystemCompatibility | NovaSeq5000,HiSeq2000,HiScanSQ,MiSeqFGx |
| VariantClass | SingleNucleotidePolymorphisms(SNPs),GeneFusions,ShortTandemRepeats(STRs) |
| SpecializedSampleTypes | LowInput |
| Technology | Sequencing |
| SpeciesCategory | Human |
| Method | AmpliconSequencing,TargetedDNASequencing |
ForenSeqDNASignaturePrepKitWorkflow:
TheForenSeqDNASignaturePrepKitispartofafullyintegrated,sample-to-answersolution,includinglibrarypreparation,DNAsequencingplatform,anddataanalysissoftwarespecificallydesignedforforensicgenomics.ForenSeqDNASignaturePrepKitForensicLociandInvestigativeWorkflow:
With~200geneticmarkersinasingleworkflow,theMiSeqFGxSystemoffersthemostcomprehensivemultiplexofSTRsandSNPsandthemoststraightforwardpathtohumanidentification.
