ProductHighlights:
TheNexteraDNAFlexLibraryPrepKitoffersflexibilityformanywhole-genomesequencingapplications.
- FastestIlluminalibraryprepworkflow,with~3.5hourstotaltime
- Flexibilitytoaccommodatevariationsinsampletype,DNAinputamount,andapplication
- Optimizedlibraryprepperformance,generatingreliableresults
SaveTimeandResources
TheNexteraDNAFlexLibraryPrepKitusesafast,user-friendlyworkflow.On-beadtagmentationchemistryreducestotallibrarypreptimeto~3.5hours,fromDNAextractiontolibrarynormalization.
SimplifyLabOperations
TheNexteraDNAFlexworkflowsupportsabroadDNAinputrange(1–500ng),multiplesampletypes,andbothsmallandlargegenomes.TheworkflowincludesDNAextractionfromblood,saliva,ordriedbloodspots*orbacterialcolonies*.Gaintheflexibilitytosequencehumanorotherlarge,complexgenomesaswellasampliconsormicrobialspecies,allwithasinglekit.
ObtainReliableResults
Whileaccommodatingvariousstudyrequirements,theNexteraDNAFlexworkflowdeliversconsistentinsertsizes,uniformcoverage,andoptimizedperformance,regardlessofDNAinputamountorgenomesize.Thebead-basedtechnologyminimizesbiasandopportunitiesforerror,resultinginhighlyreproducIBLesequencingdata.
*Demonstratedprotocolsavailable.
Specifications:
| AssayTime | ~3.5hourstotalassaytime |
| Hands-OnTime | 1-1.5hours |
| InputQuantity | SmallGenomes:1-500ngDNA;LargeGenomes:100-500ng |
| ContentSpecifications | HumanWholeGenome,SmallWholeGenome,LargeWholeGenome |
| MechanismofAction | Bead-linkedtransposome |
| Multiplexing | Upto96availableindexes |
| SpeciesCategory | AnySpecies |
| SystemCompatibility | MiSeq,HiSeqXTen,NextSeq550,HiSeq3000,HiSeqXFive,MiSeqDxinResearchMode,MiniSeq,HiSeq2500,NovaSeq6000,HiSeq4000 |
| VariantClass | SingleNucleotidePolymorphisms(SNPs),GeneFusions,LossofHeterozygosity(LOH),SomaticVariants,ChromosomalAbnormalities,GermlineVariants,StructuralVariants,Insertions-Deletions(indels),CopyNumberVariants(CNVs) |
| Method | Whole-GenomeSequencing |
| Technology | Sequencing |
| AutomationCapABIlity | LiquidHandlingRobots |
